In this article we will discuss about the recombinant dna technology. The principle of recombinant dna technology involved four steps. The resulting dna is called the recombinant dna, chimera or recombinant vector. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Ligase enzyme is the joining enzyme that joins the vector dna with gene of interest. Steps in recombinant dna technology or rdna technology youtube. Recombinant dna technology lecture basics of recombinant. Recombinant dna refers to the creation of new combinations of dna segments that are not found together in nature. This recombinant dna technology lecture explains about the basics of recombinant dna technology processes and the mechanism behind recombinant dna production. Gene cloning requirements, principle, steps, applications. In the process, restriction enzymes functions as scissors for cutting dna molecules. Since the focus of all genetics is the gene, the fundamental goal of laboratory geneticists is to isolate, characterize. Biotechnology which is synonymous with genetic engineering or recombinant dna rdna is an industrial process that uses the scientific research on dna for practical applications.
Recombinant dna, molecules of dna from two different species that are inserted into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture, and industry. Pdf concepts and tools for recombinant dna technology. In genetics recombinant dna technology has many uses e. If youre seeing this message, it means were having trouble loading external resources on our website. Recombinant dna technology development and applications b. Pdf recombinant dna technology and genetic engineering. Vaccine generated using recombinant dna technology is called recombinant vaccine. Biomedical importance of recombinant dna technology 3. Isolation of dna gene of interest fragments to be cloned. Recombinant dna technology is better referred to as genetic engineering.
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